Difficulties detecting miRNA-203 in human whole saliva by the use of PCR

OBJECTIVES Oral Lichen Planus (OLP) is a chronic disease of the oral mucosa, and according to the WHO also a pre malignant condition. Micro-RNAs are short non coding RNAs capable of regulating mRNA expression. MiRNA:scan be detected in tissue, blood and human whole saliva, HWS, and recently we have shown miR-203 to be up-regulated in tissue from OLP lesions. STUDY DESIGN In order to see whether mRNA as well asmiR-203 could be detected also in HWS, saliva from healthy controls and patients with OLP were analysed using two different PCR methods. RESULTS RESULTS showed low mRNA and miRNAlevels in general in HWS samples, making it hard to generate conclusive results. CONCLUSIONS In order to make HWS a valuable source for different analyses, more sensitive PCR techniques capable of detecting very low levels of mRNAand miRNAas well as more efficient methods for extraction of RNA are needed.